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mcsf growth factor  (Shenandoah Biotechnology)

 
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    Structured Review

    Shenandoah Biotechnology mcsf growth factor
    Male and female bone marrow-derived macrophages were cultured with 25 ng/mL <t>MCSF</t> alone or 25 ng/mL MCSF and 100 <t>ng/mL</t> <t>RANKL</t> for 3 days. (A) Micrographs of macrophages and osteoclasts prior to lysis and RNA extraction. Cells cultured with RANKL demonstrated multinucleation and increased size. (B) Micrographs of TRAP-stained osteoclast differentiated under conditions matched to RNA source cells.
    Mcsf Growth Factor, supplied by Shenandoah Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcsf+growth+factor/macrophage+colony+stimulating+factor/pmc09922499-309-44-46
    Average 90 stars, based on 1 article reviews
    mcsf growth factor - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "RANK signaling in osteoclast precursors results in a more permissive epigenetic landscape and sexually divergent patterns of gene expression"

    Article Title: RANK signaling in osteoclast precursors results in a more permissive epigenetic landscape and sexually divergent patterns of gene expression

    Journal: PeerJ

    doi: 10.7717/peerj.14814

    Male and female bone marrow-derived macrophages were cultured with 25 ng/mL MCSF alone or 25 ng/mL MCSF and 100 ng/mL RANKL for 3 days. (A) Micrographs of macrophages and osteoclasts prior to lysis and RNA extraction. Cells cultured with RANKL demonstrated multinucleation and increased size. (B) Micrographs of TRAP-stained osteoclast differentiated under conditions matched to RNA source cells.
    Figure Legend Snippet: Male and female bone marrow-derived macrophages were cultured with 25 ng/mL MCSF alone or 25 ng/mL MCSF and 100 ng/mL RANKL for 3 days. (A) Micrographs of macrophages and osteoclasts prior to lysis and RNA extraction. Cells cultured with RANKL demonstrated multinucleation and increased size. (B) Micrographs of TRAP-stained osteoclast differentiated under conditions matched to RNA source cells.

    Techniques Used: Derivative Assay, Cell Culture, Lysis, RNA Extraction, Staining

    (A) Volcano plots depicting genes meeting fold change and p -value thresholds in MCSF and MCSF+RANKL treated cells. Differentials with −log10 transformed adjusted p -values of six and greater are plotted at the same level. Full volcano plots are included in . (B) MCSF-specific, MCSF+RANKL-specific, and common pathways altered during osteoclastogenesis. Pathways are listed in .
    Figure Legend Snippet: (A) Volcano plots depicting genes meeting fold change and p -value thresholds in MCSF and MCSF+RANKL treated cells. Differentials with −log10 transformed adjusted p -values of six and greater are plotted at the same level. Full volcano plots are included in . (B) MCSF-specific, MCSF+RANKL-specific, and common pathways altered during osteoclastogenesis. Pathways are listed in .

    Techniques Used: Transformation Assay

    Related Articles

    Derivative Assay:

    Article Title: RANK signaling in osteoclast precursors results in a more permissive epigenetic landscape and sexually divergent patterns of gene expression
    Article Snippet: Osteoclast precursors from male and female mice were divided into two treatment groups: one group of cells (macrophages) were maintained in α -MEM with 25 ng/mL MCSF for 72 h; the other group of cells (osteoclasts), were maintained in α -MEM with 25 ng/mL MCSF (200-08; Shenandoah Biotechnology, Warminster, PA, USA) and 100 ng/mL RANKL (200-04; Shenandoah Biotechnology, Warminster, PA, USA) for 72 h. Media were refreshed at 48 h. At the conclusion of the culture period, cells were lysed in TRI reagent and RNA was extracted using the Direct-zol RNA miniprep kit (R2051; Zymo Research, Irvine, CA, USA).

    Cell Culture:

    Article Title: RANK signaling in osteoclast precursors results in a more permissive epigenetic landscape and sexually divergent patterns of gene expression
    Article Snippet: Osteoclast precursors from male and female mice were divided into two treatment groups: one group of cells (macrophages) were maintained in α -MEM with 25 ng/mL MCSF for 72 h; the other group of cells (osteoclasts), were maintained in α -MEM with 25 ng/mL MCSF (200-08; Shenandoah Biotechnology, Warminster, PA, USA) and 100 ng/mL RANKL (200-04; Shenandoah Biotechnology, Warminster, PA, USA) for 72 h. Media were refreshed at 48 h. At the conclusion of the culture period, cells were lysed in TRI reagent and RNA was extracted using the Direct-zol RNA miniprep kit (R2051; Zymo Research, Irvine, CA, USA).

    Lysis:

    Article Title: RANK signaling in osteoclast precursors results in a more permissive epigenetic landscape and sexually divergent patterns of gene expression
    Article Snippet: Osteoclast precursors from male and female mice were divided into two treatment groups: one group of cells (macrophages) were maintained in α -MEM with 25 ng/mL MCSF for 72 h; the other group of cells (osteoclasts), were maintained in α -MEM with 25 ng/mL MCSF (200-08; Shenandoah Biotechnology, Warminster, PA, USA) and 100 ng/mL RANKL (200-04; Shenandoah Biotechnology, Warminster, PA, USA) for 72 h. Media were refreshed at 48 h. At the conclusion of the culture period, cells were lysed in TRI reagent and RNA was extracted using the Direct-zol RNA miniprep kit (R2051; Zymo Research, Irvine, CA, USA).

    RNA Extraction:

    Article Title: RANK signaling in osteoclast precursors results in a more permissive epigenetic landscape and sexually divergent patterns of gene expression
    Article Snippet: Osteoclast precursors from male and female mice were divided into two treatment groups: one group of cells (macrophages) were maintained in α -MEM with 25 ng/mL MCSF for 72 h; the other group of cells (osteoclasts), were maintained in α -MEM with 25 ng/mL MCSF (200-08; Shenandoah Biotechnology, Warminster, PA, USA) and 100 ng/mL RANKL (200-04; Shenandoah Biotechnology, Warminster, PA, USA) for 72 h. Media were refreshed at 48 h. At the conclusion of the culture period, cells were lysed in TRI reagent and RNA was extracted using the Direct-zol RNA miniprep kit (R2051; Zymo Research, Irvine, CA, USA).

    Staining:

    Article Title: RANK signaling in osteoclast precursors results in a more permissive epigenetic landscape and sexually divergent patterns of gene expression
    Article Snippet: Osteoclast precursors from male and female mice were divided into two treatment groups: one group of cells (macrophages) were maintained in α -MEM with 25 ng/mL MCSF for 72 h; the other group of cells (osteoclasts), were maintained in α -MEM with 25 ng/mL MCSF (200-08; Shenandoah Biotechnology, Warminster, PA, USA) and 100 ng/mL RANKL (200-04; Shenandoah Biotechnology, Warminster, PA, USA) for 72 h. Media were refreshed at 48 h. At the conclusion of the culture period, cells were lysed in TRI reagent and RNA was extracted using the Direct-zol RNA miniprep kit (R2051; Zymo Research, Irvine, CA, USA).

    Transformation Assay:

    Article Title: RANK signaling in osteoclast precursors results in a more permissive epigenetic landscape and sexually divergent patterns of gene expression
    Article Snippet: Osteoclast precursors from male and female mice were divided into two treatment groups: one group of cells (macrophages) were maintained in α -MEM with 25 ng/mL MCSF for 72 h; the other group of cells (osteoclasts), were maintained in α -MEM with 25 ng/mL MCSF (200-08; Shenandoah Biotechnology, Warminster, PA, USA) and 100 ng/mL RANKL (200-04; Shenandoah Biotechnology, Warminster, PA, USA) for 72 h. Media were refreshed at 48 h. At the conclusion of the culture period, cells were lysed in TRI reagent and RNA was extracted using the Direct-zol RNA miniprep kit (R2051; Zymo Research, Irvine, CA, USA).

    Isolation:

    Article Title: RANK signaling in osteoclast precursors results in a more permissive epigenetic landscape and sexually divergent patterns of gene expression
    Article Snippet: Osteoclast precursors from male and female mice were divided into two treatment groups: one group of cells (macrophages) were maintained in α -MEM with 25 ng/mL MCSF for 72 h; the other group of cells (osteoclasts), were maintained in α -MEM with 25 ng/mL MCSF (200-08; Shenandoah Biotechnology, Warminster, PA, USA) and 100 ng/mL RANKL (200-04; Shenandoah Biotechnology, Warminster, PA, USA) for 72 h. Media were refreshed at 48 h. At the conclusion of the culture period, cells were lysed in TRI reagent and RNA was extracted using the Direct-zol RNA miniprep kit (R2051; Zymo Research, Irvine, CA, USA).



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    mcsf growth factor  (Shenandoah Biotechnology)
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    Shenandoah Biotechnology mcsf growth factor
    Male and female bone marrow-derived macrophages were cultured with 25 ng/mL <t>MCSF</t> alone or 25 ng/mL MCSF and 100 <t>ng/mL</t> <t>RANKL</t> for 3 days. (A) Micrographs of macrophages and osteoclasts prior to lysis and RNA extraction. Cells cultured with RANKL demonstrated multinucleation and increased size. (B) Micrographs of TRAP-stained osteoclast differentiated under conditions matched to RNA source cells.
    Mcsf Growth Factor, supplied by Shenandoah Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcsf+growth+factor/macrophage+colony+stimulating+factor/pmc09922499-309-44-46
    Average 90 stars, based on 1 article reviews
    mcsf growth factor - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Male and female bone marrow-derived macrophages were cultured with 25 ng/mL MCSF alone or 25 ng/mL MCSF and 100 ng/mL RANKL for 3 days. (A) Micrographs of macrophages and osteoclasts prior to lysis and RNA extraction. Cells cultured with RANKL demonstrated multinucleation and increased size. (B) Micrographs of TRAP-stained osteoclast differentiated under conditions matched to RNA source cells.

    Journal: PeerJ

    Article Title: RANK signaling in osteoclast precursors results in a more permissive epigenetic landscape and sexually divergent patterns of gene expression

    doi: 10.7717/peerj.14814

    Figure Lengend Snippet: Male and female bone marrow-derived macrophages were cultured with 25 ng/mL MCSF alone or 25 ng/mL MCSF and 100 ng/mL RANKL for 3 days. (A) Micrographs of macrophages and osteoclasts prior to lysis and RNA extraction. Cells cultured with RANKL demonstrated multinucleation and increased size. (B) Micrographs of TRAP-stained osteoclast differentiated under conditions matched to RNA source cells.

    Article Snippet: Osteoclast precursors from male and female mice were divided into two treatment groups: one group of cells (macrophages) were maintained in α -MEM with 25 ng/mL MCSF for 72 h; the other group of cells (osteoclasts), were maintained in α -MEM with 25 ng/mL MCSF (200-08; Shenandoah Biotechnology, Warminster, PA, USA) and 100 ng/mL RANKL (200-04; Shenandoah Biotechnology, Warminster, PA, USA) for 72 h. Media were refreshed at 48 h. At the conclusion of the culture period, cells were lysed in TRI reagent and RNA was extracted using the Direct-zol RNA miniprep kit (R2051; Zymo Research, Irvine, CA, USA).

    Techniques: Derivative Assay, Cell Culture, Lysis, RNA Extraction, Staining

    (A) Volcano plots depicting genes meeting fold change and p -value thresholds in MCSF and MCSF+RANKL treated cells. Differentials with −log10 transformed adjusted p -values of six and greater are plotted at the same level. Full volcano plots are included in . (B) MCSF-specific, MCSF+RANKL-specific, and common pathways altered during osteoclastogenesis. Pathways are listed in .

    Journal: PeerJ

    Article Title: RANK signaling in osteoclast precursors results in a more permissive epigenetic landscape and sexually divergent patterns of gene expression

    doi: 10.7717/peerj.14814

    Figure Lengend Snippet: (A) Volcano plots depicting genes meeting fold change and p -value thresholds in MCSF and MCSF+RANKL treated cells. Differentials with −log10 transformed adjusted p -values of six and greater are plotted at the same level. Full volcano plots are included in . (B) MCSF-specific, MCSF+RANKL-specific, and common pathways altered during osteoclastogenesis. Pathways are listed in .

    Article Snippet: Osteoclast precursors from male and female mice were divided into two treatment groups: one group of cells (macrophages) were maintained in α -MEM with 25 ng/mL MCSF for 72 h; the other group of cells (osteoclasts), were maintained in α -MEM with 25 ng/mL MCSF (200-08; Shenandoah Biotechnology, Warminster, PA, USA) and 100 ng/mL RANKL (200-04; Shenandoah Biotechnology, Warminster, PA, USA) for 72 h. Media were refreshed at 48 h. At the conclusion of the culture period, cells were lysed in TRI reagent and RNA was extracted using the Direct-zol RNA miniprep kit (R2051; Zymo Research, Irvine, CA, USA).

    Techniques: Transformation Assay